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THETM His Tag Antibody [Biotin], mAb, Mouse

Catalog No. Size Price Figures
A00613-100 ug
 		100 ug
$ 410.00
MSDS:  20080118034804  (PDF)
MSDS:  20080213024757  (PDF)
COA:  A00613_10L000226  (PDF)
COA:  A00613_10L000226  (DOC)

EXAMPLE:
Mouse Anti His-tag [Biotin] mAb
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Full Name
THETM His Tag Antibody [Biotin], mAb, Mouse
Alias THETM His Tag Antibody [Biotin], mAb, Mouse
DescriptionGenScript THETM His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated THETM His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of His in fusion proteins.
Ig SubclassIgG1, k
StorageThe antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
SpecificityGenScript THETM His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Concentration0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Clone6G2A9
ImmunogenA synthetic peptide (HHHHHH) coupled to KLH
Purification MethodProtein A affinity column
ReconstitutionReconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
ApplicationsWorking concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.02-0.2 µg/ml
Western blot: 0.5-1.0 µg/ml
Other applications: user-optimized
ConjugationBiotin
Host SpeciesMouse
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical ManualTM0589, TM0590, TM0591, TM0592, TM0593
* For Non-Clinical Research Use Only *
Related Products:
CodeNameSizePrice 
A00174antibody : His-tag Antibody, pAb, Rabbit40 ug
$ 50.00
200 ug
$ 225.00
A00186 antibody : THETM His Tag Antibody, mAb, Mouse40 ug
$ 180.00
100 ug
$ 375.00
200 ug
$ 675.00
A00612antibody : THETM His Tag Antibody [HRP], mAb, Mouse100 ug
$ 410.00
M00091 Immunology Reagents: Streptavidin-HRP1 mg
$ 89.00




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